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李利教授
Department of Chemical and Biomolecular Engineering
The Ohio State University
ABSTRACT
Nucleic acid therapeutics including small interfering RNA, microRNA, messenger RNA, and DNA
have great potential for disease treatment. However, a major limiting factor is the ability to deliver well-
defined amounts of these relatively large and negatively charged molecules into target tissues and
cells. A variety of cell transfection techniques have been developed for in vivo gene delivery, including
viral vectors and synthetic nanoparticles. But they suffer from severe immunogenicity, poor efficacy,
and/or high cost. Recently, cell-secreted vesicles that encapsulate genetic and proteomic materials
have emerged as promising therapeutic agents However, only a few cell types such as multipotent
stem cells are found to secret high numbers of exosomes that exhibit immunosuppressive activity.
Here we show the development of a new technology platform, nanochannel electroporation for highly
effective cell transfection and vesicle secretion. The potential of those transfected cells and their
secreted vesicles is demonstrated in several frontier medical fields including non-viral generation of
induced neurons for stroke recovery and induced endothelial cells for wound healing, therapeutic
neutrophils for targeted rheumatoid arthritis treatment, and therapeutic exosomes for glioblastoma
treatment. |
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